Phosphoramidites

Phosphoramidites are the important class of intermediates for polynucleotide synthesis. Structurally phosphoramidites are the amides of trivalent phosphorus acids [2-Cyanoethoxy diisopropylamino phosphino group]. The phosphoramidites are sensitive towards water and oxygen. The main degradation involves oxidation, hydrolysis, and elimination of acrylonitrile and autocatalytic acrylonitrile-induced formation of cyanoethyl phosphonoamidates. Isolation and purification require special techniques and attention. Sapala has developed techniques for the synthesis of a wide range of nucleoside phosphoramidites with excellent purity.

  • Orthogonally protected 3’-phosphoramidites of nucleosides
  • 2’-Deoxy-5’-DMT-3’-phosphoramidites of nucleosides
  • Orthogonally protected 5’-phosphoramidites
  • Phosphoramidites of various sugar and base modified nucleosides

Phosphoramidites of nucleoside with a linker, such as an amino alkyl or an alkyl amide linker became popular in recent years. The following group of phosphoramidites can be made upon request.

  • Biotin phosphoramidites
  • Dye phosphoramidites
  • Sugar phosphoramidites
Product No. Structure IUPAC Name CAS No. Specification & MSDS
SPR-0001 phosphoramidites ligands (2R,3S)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite --------  phosphoramidites of nucleosides
SPR-0002 phosphoramidites DNA synthesis (2R,3S,5S)-5-(4-benzamido-2-oxopyrimidin-1(2H)-yl)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite --------  phosphoramidites RNA synthesis
SPR-0003 phosphoramidites chemistry (2R,3S,5S)-5-(6-benzamido-9H-purin-9-yl)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite --------  Dye phosphoramidites
SPR-0004 Biotin phosphoramidites (2R,3R,4R,5R)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-hydroxy-4-methyl-tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite --------  Sugar phosphoramidites
SPR-0005 Orthogonally protected phosphoramidites (2R,3R,4R,5R)-5-(6-benzamido-9H-purin-9-yl)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxy-4-methyl-tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite --------  autocatalytic acrylonitrile-induced